Evaluation of rapid carbohydrate degradation tests for identification of pathogenic Neisseria.

نویسندگان

  • D J Pizzuto
  • J A Washington
چکیده

A total of 156 clinical isolates were tested by the modified rapid fermentation test, the BACTEC Neisseria differentiation kit, and the cystine-Trypticase agar method. The modified rapid fermentation test and BACTEC methods accurately identified at least 95% of 101 strains of N. gonorrhoeae tested and at least 91% of 45 strains of N. meningitidis tested within 4 h. Overall, the cystine-Trypticase agar method was the most accurate (97%) but required as long as 48 h of incubation. The data presented appear to show that rapid carbohydrate degradation tests can provide reliable and specific identification results.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Rapid identification of pathogenic species of Neisseria by carbohydrate degradation tests. Importance of glucose in media used for preparation of inocula.

Pathogenic species of Neisseria were identified more readily by carbohydrate degradation tests when 0.5% glucose was used in media from which inocula for the test were obtained. This improved the performance of both non-growth and growth-dependent methods for these tests. One of the three techniques used a non-nutrient buffered salt solution and depended on the presence of preformed enzymes. Th...

متن کامل

Evaluation of the RIM-N, Gonochek II, and Phadebact systems for the identification of pathogenic Neisseria spp. and Branhamella catarrhalis.

Methods for identifying Neisseria spp. include conventional and modified carbohydrate degradation procedures, chromogenic enzyme substrate tests, and immunologic coagglutination tests for Neisseria gonorrhoeae. In this study, we evaluated the abilities of the RIM-N carbohydrate degradation system (American MicroScan, Campbell, Calif.), the Gonochek II enzymatic identification system (Du Pont Co...

متن کامل

Rapid enzyme system for the identification of pathogenic Neisseria spp.

Gonochek II is a combination of three enzyme substrates in one tube which will give a rapid identification (30 min) of those pathogenic Neisseria spp. which can be isolated on Thayer Martin or similar selective media. Eighty isolates were tested by Gonochek II and a carbohydrate utilization method; total agreement was achieved between the two methods.

متن کامل

Rapid carbohydrate fermentation test for confirmation of the pathogenic Neisseria using a Ba(OH)2 indicator.

The Ba(OH)2 indicator system was demonstrated to be a practical procedure in assisting clinical bacteriologists in the accurate and rapid identification of the pathogenic Neisseria from clinical specimens. This system measured the release of CO2, resulting from the metabolism of fermentable carbohydrate, as the precipitated BaCO3, by means of a spectrophotometer, The method was uncomplicated an...

متن کامل

Carbohydrate fermentation plate medium for confirmation of Neisseria species.

A carbohydrate fermentation plate medium is described for rapid and reliable confirmation of Neisseria gonorrhoeae, N. meningitidis, and other Neisseria species. The medium is based on a modification of NYC (U.S. Patent 3,846,241) medium, originally designed for the isolation of pathogenic Neisseria (3). A total of 715 clinical isolates were tested for their carbohydrate fermentation reactions ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 11 4  شماره 

صفحات  -

تاریخ انتشار 1980